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UC Davis Comprehensive Cancer Center

UC Davis Comprehensive Cancer Center

Flow Cytometry

Technical Director: Bridget McLaughlin

Video: Bridget McLaughlin, technical director of the Flow Cytometry Shared Resource at UC Davis
Comprehensive Cancer Center, describes the vast capabilities of the resource to assist cancer researchers.

The mission of the UC Davis Flow Cytometry Shared Resource Laboratory is to provide access to expertise and instrumentation for analytical flow cytometry, cell sorting and laser scanning cytometry to all researchers at UC Davis, including members of the UC Davis Comprehensive Cancer Center, and to biotech companies and other research organizations.

Research support for flow cytometry and cell sorting

Bridget McLaughlin and Jonathan Van Dyke © UC RegentsCell sorting and analytical cytometers are located at three sites in Davis and Sacramento for research use on a recharge basis. The Davis and Sacramento labs are managed by Bridget McLaughlin, technical director, and Jonathan Van Dyke, technical manager, who operate the cell sorters for assisted sorting on an appointment basis and provide training to investigators in acquiring data on the analytic cytometers. With a combined 30-plus years of flow cytometry expertise, we can advise on experiment design for flow cytometry, provide training to enable independent use of the analytical cytometers, and familiarize investigators with popular data analysis software tools such as BD Diva and CellQuest, FlowJo, ModFit and iBrowser.

UC Davis campus

Davis Campus Shared Flow Cytometry Resource
3425 Tupper Hall, Health Sciences Complex, Davis, Phone: 530-752-7205

  • Cell sorter: 10 color Cytomation MoFlo high speed cell sorter
  • Analytic cytometers:
       Cytek xP5 (FACScan) 5-color cytometer
       BD LSRII 17 color cytometer
  • Laser Scanning Cytometer:
       3-laser, 4-color solid substrate, microscope-based, inverted optic scanning cytometer for histological specimens, cytospin slides and tissue culture dishes
  • Fluidigm BioMark: microscale single-cell real-time gPCR analyzer for targeted gene transcription, genotyping and SNIP detection
UC Davis Medical Center

Sacramento Health System Shared Flow Cytometry Resource
Institute for Regenerative Cures, 2921 Stockton Blvd., Suite 1670, Sacramento,
Phone: 916-703-9307

  • Cell sorter: 16-color BD inFlux high speed cell sorter in HEPA enclosure
  • Analytic cytometers:
       BD Fortessa 18-color cytometer
       Stratedigm 12-color S1000EX cytometer

Sacramento Health System Satellite Facility
Research Bldg. III, 4645 2nd Ave., Room 3154, Sacramento

  • BD FACScan 3-color cytometer
The Flow Cytometry Shared Resource offers the following general services:
  • Consultation about the experiment design and preparation of cells for analysis
  • Training on use of equipment, associated computer programs, and interpretation of data
  • Scheduling of instrument usage and billing
  • Acquisition of data and analysis on a fee-for-service basis
Specific services currently include:
  • Cell sorting and analysis in 16 colors
  • One to 18-color analysis of fluorescence-labeled cells
  • Intracellular protein detection including cytokines and viral proteins
  • Analysis of green fluorescent protein expression
  • Apoptosis assays
  • DNA content for cell cycle distribution
  • Intracellular calcium levels
  • Mitochondrial membrane potential
  • Analysis of fluorescent in situ hybridization (FISH)
Flow Cytometry Shared Resource recharge rates:

Our current hourly rates are shown below; please note that Comprehensive Cancer Center members receive a lower, subsidized rate for cancer-related research.

Instrument or service
Non-member
with core
operator
assistance
rate
Non-member
self-operated
rate
Cancer Center
member
with core
operator
assistance
rate
Cancer Center
member self-
operated
rate
Becton Dickinson FACScan - Davis $118 $67 $83 $47
Becton Dickinson FACScan - Sacramento $118 $67 $83 $47
Becton Dickinson Fortessa, Sacramento $134 $96 $94 $68
Becton Dickinson LSR II, Davis $132 $94 $93 $66
Consulting Services $60 N/A $60 N/A
Cytomation MoFlo Cell Sorter, Davis $151 $114 $106 $80
Stratedigm S1000EX Cytometer, Sacramento $134 $84 $94 $66
iCys® Research Imaging Cytometer, Davis $86 $33 $60 $23
inFlux Cell Sorter, Sacramento $151 $114 $106 $80

 

To learn more or to make an appointment for cell sorting or instrument training, contact:

Bridget McLaughlin

Jonathan Van Dyke

Bridget McLaughlin
Technical Director
Phone: 530-752-7205
Email

Jonathan Van Dyke
Technical Manager
Phone: 916-703-9307
Email

 

Shared Resources is an online service provided to support core cancer research. Please log in and create a secure profile to view our online reservation calendar.

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