Wenbin Deng, Ph.D.
Professor
653B Shriner's Hospital
Sacramento Campus
Ph: 916-453-2287
e-mail

The Deng Lab is interested in studying neural development and disease. We investigate how stem cells differentiate and give rise to neurons and glia, and then the grey matter and white matter and connected regions, and how variations in basic programs of development underlie nervous system disorders. We use mouse and human model systems, genetic fate mapping strategies, cellular and behavioral phenotyping, and high throughput library or omics techniques. Current projects focus on the regulation of neural stem cell identity and differentiation using human induced pluripotent stem cells (hiPSC) differentiated into neurons and glia and their interconnected networks in vitro, genetic and epigenetic regulation of neural development and disease in hiPSC models or using mouse models, and stem cell responses to environmental perturbations, including hypoxia-ischemia, oxidative stress, and inflammation. We then use these models and technology platforms to identify therapeutic targets and intervention strategies.

Jiang P, Chen C, Liu X, Pleasure DE, Liu Y, Deng W. (2016) Human iPSC-derived immature astroglia promote oligodendrogenesis by increasing TIMP-1 secretion. Cell Reports, 15 (6): 1303-1315. doi: 10.1016/j.celrep.2016.04.011. PMID: 27134175.

Daugherty DJ, Chechneva OV, Mayrhofer F, Deng W. (2016) The hGFAP-driven conditional TSPO knockout is protective in a mouse model of multiple sclerosis. Sci. Reports, 2016 Mar 1;6:22556. doi: 10.1038/srep22556. PMID: 26925573. http://www.nature.com/articles/srep22556.

Zhang M, Biswas S, Deng W*, Yu H*. (2016) The crystal structure of monovalent streptavidin. Sci. Reports, 2016 Dec 1; 6:35915. DOI: 10.1038/srep35915. http://www.nature.com/articles/srep35915.
*Co-correspondence authors.

Yechikov S, Copaciu R, Gluck JM, Deng W, Chiamvimonvat N, Chan JW, Lieu DK. (2016) Same-single-cell analysis of pacemaker-specific markers in human induced pluripotent stem cell-derived cardiomyocyte subtypes classified by electrophysiology. Stem Cells. 2016 Jul 19. doi: 10.1002/stem.2466. PMID: 27434649.

Chechneva OV, Deng W. (2016) Mitochondrial translocator protein (TSPO), astrocytes and neuroinflammation. Neural Regen Res 11(7):1056-1057. PMID: 27630677.

Jiang P, Deng W. (2016) Regenerating white matter using human iPSC-derived immature astroglia. Neurogenesis (Austin). 2016 Sep 8; 3(1):e1224453. doi: 10.1080/23262133.2016.1224453. eCollection 2016. PMID: 27652287.

Tian Z, Guo F, Biswas S*, Deng W*. (2016) Rationale and methodology of reprogramming for generation of induced pluripotent stem cells and induced neural progenitor cells. Int. J. Mol. Sci. 17 (4), 594; doi:10.3390/ijms17040594. *Co-correspondence authors.

Zhang M, Biswas S, Qin X, Gong W, Deng W*, Yu H*. (2016) Does Notch play a tumor suppressor role across diverse carcinomas? Cancer Med. 2016 Aug; 5(8): 2048-2060. doi: 10.1002/cam4.731. PMID: 27228302. *Co-correspondence authors.

Liu X, Shen Y, Pleasure DE, Deng W. (2016) The vulnerability of thalamocortical circuitry to hypoxic-ischemic injury in a mouse model of periventricular leukomalacia. BMC Neurosci, 17 (2), DOI 10.1186/s12868-015-0237-4.

Zhai L, Wang L, Teng F, Zhou L, Zhang W, Xiao J, Liu Y, Deng W. (2016) Argonaute and argonaute-bound small RNAs in stem cells. Int. J. Mol. Sci. 17 (2): 208. doi: 10.3390/ijms17020208

Chen C, Chan A, Wen H, Chung SH,Deng W*, Jiang P*. (2015) Stem and progenitor cell-derived astroglia therapies for neurological diseases. Trends Mol Med, 21 (11): 715-729.*Co-correspondence authors.

Li S, Xue H, Wu J, Rao MS, Kim D, Deng W,  Liu Y. (2015) Human iPSC NEUROG2 dual reporter knockin lines generated by the CRISPR/Cas9 system. Stem Cells Dev, 24 (24): 2925-2942. doi:10.1089/scd.2015.0131. PMID: 26414932.

Chung SH, Biswas S, Selvaraj V, Liu X-B, Sohn J, Jiang P, Chen C, Chmilewsky F, Marzban H, Horiuchi M, Pleasure DE, Deng W. (2015) The p38α mitogen activated protein kinase is a key regulator of myelination and remyelination in the CNS. Cell Death & Dis, (2015) 6, e1748. doi:10.1038/cddis.2015.119.

Sohn J, Orosco L, Guo F, Chung SH, Bannerman P, Mills Ko E, Zarbalis K, Deng W, Pleasure DE. (2015) The subventricular zone continues to generate corpus callosum and rostral migratory stream astroglia in normal adult mice. J Neurosci, 35 (9):3756-3763.

Hammond E, Lang J, Maeda Y, Pleasure D, Angus-Hill M, Xu J, Horiuchi M, Deng W, Guo F. (2015) The Wnt effector transcription factor 7-like 2 positively regulates oligodendrocyte differentiation in a manner independent of Wnt/beta-catenin signaling. J Neurosci, 35 (12): 5007-5022.

Xiao J, Yang R, Yang L, Fan X, Liu W, Deng W. (2015) Kirenol attenuates experimental autoimmune encephalomyelitis by inhibiting differentiation of Th1 and Th17 cells and inducing apoptosis of effector T cells. Sci. Reports, 5, 9022; DOI:10.1038/srep09022 (2015).
http://www.nature.com/srep/2015/150312/srep09022/full/srep09022.html

Chechneva OV, Deng W. (2015) Empowering sonic hedgehog to rescue brain cells after ischemic stroke.Neural Regen Res, 10 (3): 360-362. doi: 10.4103/1673-5374.153677. PMID: 25878576 [PubMed].

Qin X, Xu H, Gong W, Deng W. (2015) The tumor cytosol miRNAs, fluid miRNAs and exosome miRNAs in cancer. Front. Oncol. 4: 357. doi: 10.3389/fonc.2014.00357 (2015). PMID: 25601903 [PubMed].

Xiao J, Yang R, Biswas S, Qin X, Zhang M, Deng W. (2015) Mesenchymal stem cells and induced pluripotent stem cells as therapies for multiple sclerosis. Int. J. Mol. Sci. 16: 9283-9302; doi:10.3390/ijms16059283.

Liu Y, Deng W. (2015) Reverse engineering human neurodegenerative disease using pluripotent stem cell technology. Brain Res 941.

Chechneva OV, Mayrhofer F, Daugherty DJ, Krishnamurty RG, Bannerman P, Pleasure DE, Deng W. (2014) A smoothened receptor agonist is neuroprotective and promotes regeneration after ischemic brain injury. Cell Death & Dis, (2014) 5, e1481. doi:10.1038/cddis.2014.446 (2014).

Lo U, Selvaraj V, Plane JM, Chechneva OV, Otsu, K, Deng W. (2014) p38α (MAPK14) critically regulates the immunological response and the production of specific cytokines and chemokines in astrocytes. Sci. Reports, 4: 7405; DOI:10.1038/srep07405 (2014).
http://www.nature.com/srep/2014/141212/srep07405/full/srep07405.html

Chen C,  Jiang P, Xue H, Peterson SE, Tran HT, McCann AE, Parast MM, Li S, Pleasure DE, Laurent LC, Loring JF, Liu Y, Deng W. (2014) Role of astroglia in Down’s syndrome revealed by patient-derived human-induced pluripotent stem cells. Nature Communications . 5:4430 doi: 10.1038/ncomms5430 (2014).

Jiang P, Chen C, Wang R, Chechneva OV, Chung S, Rao M, Pleasure DE, Liu Y, Zhang Q, Deng W. (2013) hESC-derived Olig2+ progenitors generate a subtype of astrocytes with protective effects against ischemic brain injury. Nature Communications, 4: 2196 doi: 10.1038/ncomms3196 (2013).
(Press Release for this paper: https://www.nature.com/articles/ncomms3196)

Daugherty DJ, Selvaraj S, Chechneva OV, Liu X, Pleasure DE, Deng W. (2013) A TSPO ligand is protective in a mouse model of multiple sclerosis. EMBO Mol Med, 5 (6): 891-903.

Jiang P, Chen C, Liu X, Selvaraj V, Liu W, Feldman DH, Liu Y, Pleasure DE, Li, RA, Deng W. (2013) Generation and characterization of spiking and nonspiking oligodendroglial precursor cells from embryonic stem cells. Stem Cells, 31 (9), doi: 10.1002/stem.1515.

Chung S, Guo F, Jiang P, Pleasure DE, Deng W. (2013) Olig2/Plp+ progenitor cells give rise to Bergmann glia in the cerebellum. Cell Death & Dis, 4:e546. doi:10.1038/cddis.2013.74. PMID: 23492777.

Liu X, Shen Y, Plane JM, Deng W. (2013) Vulnerability of premyelinating oligodendrocytes to white matter damage in neonatal brain injury. Neurosci Bulletin, 29 (2): 229–238. PubMed ID: 23456565.

Zhou B, Si W, Su Z, Deng W, Tu X, Wang Q. (2013) Transcriptional activation of the Prox1 gene by HIF-1α and HIF-2α in response to hypoxia. FEBS Lett 587 (6): 724-731.

Liu W, Deng Y, Liu Y, Gong W, Deng W. (2013) Stem cell models for drug discovery and toxicology studies. J Biochem Mol Toxicol, 27 (1): 17-27.

Sohn J, Selvaraj V, Wakayama K, Orosco L, Lee E, Crawford SE, Guo F, Lang J, Horiuchi M, Zarbalis K, Itoh T, Deng W, Pleasure DE. (2012) PEDF is a novel oligodendrogenic morphogen acting on the adult SVZ and corpus callosum. J Neurosci, 32 (35):12152-12164.

Plane JM, Grossenbache SK, Deng W. (2012) Poly-ADP-ribose polymerase-1 deletion switches mouse subventricular zone stem cells toward a glial fate. J Neurosci Res, 90 (8):1489-1506.

Shen Y, Liu X, Pleasure DE, Deng W. (2012) Axon-glia synapses are highly vulnerable to white matter injury in the developing brain. J Neurosci Res, 90 (1):105-121.

Calafiore M, Copani, A, Deng W. (2012) DNA polymerase-ß mediates the neurogenic effect of ß-amyloid protein on subventricular zone stem cells. J Neurosci Res, 90: 559-567.

Chen C, Daugherty DJ, Jiang P, Deng W. (2012) Oligodendrocyte progenitor cells derived from mouse embryonic stem cells give rise to type-1 and type-2 astrocytes in vitro. Neurosci Lett, 523 (2):180-185.

Selvaraj V, Jiang P, Chechneva O, Lo U, Deng W. (2012) Differentiating human stem cells into neurons and glial cells. Frontiers in Biosciences, 17: 65-89. The first two authors contributed equally to this work.

Liu Y, Jiang P, Deng W. (2011) Olig gene targeting in human pluripotent stem cells for motor neuron and oligodendrocyte differentiation. Nature Protocols, 6(5): 640-655. The first two authors contributed equally.

Chung S, Calafiore M, Plane JM, Pleasure D, Deng W. (2011) AIF deficiency causes reduced mitofusion-1 expression and patterned Purkinje cell degeneration. Neurobiology of Disease, 41: 445–457.

Liu W, Shen Y, Plane JM, Pleasure DE, Deng W. (2011) Neuroprotective potential of erythropoietin and its derivative carbamylated erythropoietin in periventricular leukomalacia. Experimental Neurology, 230: 227-239 (selected for COVER illustration).

Zhang Jiaping, Marco Calafiore, Yuesheng Huang, Qunli Zeng, Xiuzhen Zhang, Ronald A. Li, Deng W*, Min Zhao*. (2011) Electrically guiding migration of human induced pluripotent stem cells. Stem Cell Rev Reports, 7: 987-996. PubMed ID: 21373881. *Co-correspondence authors.

Lo K, Sun Y, Landry JP, Zhu X, Deng W. (2011) Label-free detection of surface markers on stem cells by oblique-incidence reflectivity difference microscopy. BioTechniques, 50 (6): 381–388.

Wang Y, Plane JM, Jiang P, Zhou C, Deng W. (2011) Quiescent and active stages of endogenous adult neural stem cells: identification and characterization. Stem Cells, 29 (6):907-912.

Soundarapandian MM, Selvaraj V, Lo U, Golub MS, Feldman DH, Pleasure DE, Deng W. (2011) Zfp488 promotes oligodendrocyte differentiation of neural progenitor cells in adult mice after cuprizone-induced demyelination. Sci. Reports, 1:2 doi:10.1038/srep2 (2011). PMID: 22355521.
http://www.nature.com/srep/2011/110614/srep00002/full/srep00002.html

Chechneva OV, Mayrhofer F, Daugherty DJ, Pleasure DE, Hong J, Deng W. (2011) Low dose dextromethorphan attenuates moderate experimental autoimmune encephalomyelitis by inhibiting NOX2 and reducing peripheral immune cells infiltration in the spinal cord. Neurobiology of Disease, 44: 63-72.

Jantzie LL, Talos DM, Selip DB, An L, Jackson MC, Folkerth RD, Deng W, Jensen FE (2011) Developmental regulation of group I metabotropic glutamate receptors in the premature brain and their protective role in a rodent model of periventricular leukomalacia. Neuron Glia Biology, 6 (4): 277-288. Epub 2011 Dec 14.

Calafiore M, Copani, A, Deng W. (2011) DNA polymerase-ß mediates the neurogenic effect of ß-amyloid protein on subventricular zone stem cells. J Neurosci Res, doi: 10.1002/jnr.22780.

Deng W. (2010) Neurobiology of injury to the developing brain. Nature Reviews Neurology, 6 (6): 328-336.

Liu D, Ander BP, Xu H, Shen Y, Kaur P, Deng W, Sharp FR. (2010) Blood brain barrier breakdown and repair by src after thrombin-induced brain injury. Annals of Neurology, 67 (4):526-533.

Plane JM, Shen Y, Pleasure DE, Deng W. (2010) Prospects for minocycline neuroprotection. Arch Neurol, 67 (12), 1442-1448.

Deng W. (2010) Exploiting pluripotency for therapeutic gain. Panminerva Medica, 52 (2): 167-173. PubMed ID: 20517198

Deng W. (2010) AID in reprogramming: quick and efficient. BioEssays, 32 (5): 385-387.

Shen Y, Plane, JM, Deng W. (2010) Mouse models of periventricular leukomalacia. J Vis Exp, 2010 May 18;(39). pii: 1951. http://www.jove.com/index/Details.stp?ID=1951, doi: 10.3791/1951. PubMed ID: 20485263.

Jiang P, Selvaraj V, Deng W. (2010) Differentiation of embryonic stem cells into oligodendrocyte precursors. J Vis Exp, 2010 May 19;(39). pii: 1960. http://www.jove.com/index/Details.stp?ID=1960, doi: 10.3791/1960. PubMed ID: 20489683.

Jiang P, Rushing SN, Kong C, Fu J, Lieu D, Chan, C, Deng W, Li, R. (2010) Electrophysiological properties of human induced pluripotent stem cells. Am J Physiol Cell Physiol, 298 (3), C486-495.

Deng W. (2010) Induced pluripotent stem cells: paths to new medicines. EMBO reports, 11 (3), 161-165.

Selvaraj V, Plane, JM, Williams AJ, Deng W. (2010) Switching cell fate: the remarkable rise of induced pluripotent stem cells and lineage reprogramming technologies. Trends in Biotechnology, 28 (4): 214-223.

Deng W. (2010) Stem cells and drug discovery: the time has never been better. Trends in BioPharmaceutical Industry, 6 (1): 12-20. (ISSN: 1943-2518).

Selvaraj V, Soundarapandian MM, Chechneva O, Williams AJ, Sidorov MK, Soulika AM, Pleasure DE, Deng W. (2009) PARP-1 deficiency increases the severity of disease in a mouse model of multiple sclerosis. J Biol Chem, 284 (38), 26070–26084.

Deng W. (2009) PARylation: strengthening the connection between cancer and pluripotency. Cell Stem Cell, 5: 349-350.

Deng W*, Xu Y*. (2009) Genome integrity: linking pluripotency and tumorgenicity. Trends in Genetics, 25 (10): 425-427. *Co-correspondence authors.

Deng W, Pleasure J, Pleasure D. (2008) Progress in periventricular leukomalacia. Arch Neurol, 65 (10), 1291-1295.

Deng W, Neve RL, Rosenberg PA, Volpe JJ, Jensen FE. (2006) AMPA receptor composition and CREB regulate oligodendrocyte excitotoxicity. J Biol Chem, 281, 36004-36011.