Cellular and molecular mechanisms regulating arterial smooth muscle excitability The main goal of my research team is to understand the mechanisms by which local and global calcium signals regulate excitation-contraction coupling and excitation-transcription coupling in arterial smooth muscle cells during physiological and pathological conditions. To tackle these issues, we employ several state-of-the-art techniques including molecular biology, patch-clamp electrophysiology, and confocal and Total Internal Reflections Fluorescence (TIRF) microscopy. We are currently interested in determining the mechanisms by which hyperglycemia induces the activation of a novel calcium signaling modality (persistent calcium sparklets) in arterial smooth muscle. We think that activation of this signal contributes to enhanced contractility and arterial dysfunction during diabetes.
Graduate Group Affiliations:
National Institutes of Health
American Heart Association
Ph.D., University of Puerto Rico, Rio Piedras
B.S., University of Puerto Rico, Rio Piedras
Vascular Biology, University of Washington, Seattle WA
Select Recent Publications
To view a complete list of Dr. Navedo's publications, please click here.
MF Navedo*, GC Amberg. Local regulation of L-type calcium channels in arterial smooth muscle. Microcirculation 2013; 20: 290-298. *corresponding author. PMC3577995
M Nieves-Cintrón, MA Nystoriak, MP Prada, K Johnson, W Fayer, ML Dell’Acqua, JD Scott, MF Navedo. Selective downregulation of KV2.1 function contributes to enhanced arterial tone during diabetes. JBC 2015; 290: 7918-7929. PMC4367290
MA Nystoriak, M Nieves-Cintron, MF Navedo. Capturing single L-type calcium channel function with optics. Biochimica Biophysical Acta 2013; 1833: 1657-1664. PMC3574202
MA Nystoriak, M Nieves-Cintrón, PJ Nygren, SA Hinke, CB Nichols, CY Chen, JL Puglisi, L Izu, DM Bers, ML Dell'Acqua, JD Scott, LF Santana, MF Navedo. AKAP150 contributes to enhanced vascular tone by facilitating BKCa channel remodeling in hyperglycemia and diabetes. Circulation Research 2014; 114: 607-615. This article is accompanied by an editorial highlighting the novel aspects of the study. PMC3954117.
ZA Malik, IS Stein, MF Navedo, JW Hell. Mission CaMKIIγ: Shuttle calmodulin from membrane to nucleus. Cell 2014; 159: 235-237. PMC in progress.
RE Dixon, C Moreno, C Yuan, X Optiz-Araya, M Binder, MF Navedo* LF Santana*. Graded Ca2+/calmodulin-dependent coupling of voltage-gated CaV1.2 channels. eLife 4 2015. *co-corresponding author. PMC4360655