Yu-Fung Lin, Ph.D.
4144 Tupper Hall
Our long-term research goal is to understand at a molecular level how ion channels are modulated by novel mechanisms and how we may manipulate channel regulation to treat diseases and protect cells from ischemic insults. In particular, we are interested in the ATP-sensitive potassium (KATP) channel, a high-fidelity metabolic sensor which sets membrane excitability to match cellular energy demand. Defects of KATP channel functionality are associated with diseases or pathophysiological states such as hypertension, vasospasm, diabetes mellitus, congenital hyperinsulinism, seizure, dilated cardiomyopathy, and ischemic/reperfusion injury. Our current work focuses on delineating the molecular mechanism and intracellular signaling pathway responsible for functional regulation of KATP channels by cyclic-nucleotide-dependent kinases and calcium. Other lines of research in my laboratory include studies of the role of KATP channels in ischemic preconditioning and regulation of GABA(A) receptors by novel messengers.
2014 Zhang, D.M, Chai, Y., Erickson, J., Brown, J.H., Bers, D.M. and Lin, Y.F. Intracellular signaling mechanism responsible for modulation of sarcolemmal ATP-sensitive potassium channels by nitric oxide in ventricular cardiomyocytes. J. Physiol. (London) 592: 971-990.
2011 Chai, Y., Zhang, D.M. and Lin, Y.F. Activation of cGMP-dependent protein kinase stimulates cardiac ATP-sensitive potassium channels via a ROS/calmodulin/CaMKII signaling cascade. PLoS ONE 6(3): e18191, doi:10.1371/journal.pone.0018191.
2010 Chai, Y. and Lin, Y.F. Stimulation of neuronal KATP channels by cGMP-dependent protein kinase: Involvement of ROS and 5-hydroxydecanoate-sensitive factors in signal transduction. Am. J. Physiol. Cell Physiol., 298: C875-C892. Epub 2010 Jan 6.
2009 Chen, T.Y., Lin, Y.F. and Zheng, J. Electrophysiological measurements of membrane proteins, T. Jue (ed), Fundamental Concepts in Biophysics, Vol. 1, Series: Handbook of Modern Biophysics, Humana Press, Totowa, NJ.
2008 Chai, Y. and Lin, Y.F. Dual regulation of the ATP-sensitive potassium channel by activation of cyclic GMP-dependent protein kinase. Pflügers Arch.-European J. Physiol. 456: 897-915. Epub 2008 Jan 30.
2008 Lin, Y.F. and Chai, Y. Modulation of the ATP-sensitive potassium channel by extracellular signal-regulated kinase-mediated phosphorylation. Neuroscience 152: 371-380. Epub 2008 Jan 9.
2007 Misaki, N., Mao, X., Lin, Y.F., Suga, S., Li, G.H., Liu, Q., Chang, Y., Wang, H, Wakui, M. and Wu, J. Iptakalim, a vascular KATP channel opener, closes rat pancreatic beta-cell KATP channels and increases insulin release. J. Pharmacol. Exp. Ther. 322: 871-878. Epub 2007 May 23.
2007 Mao, X., Chai, Y. and Lin, Y.F. Dual regulation of ATP-sensitive potassium channels by caffeine. Am. J. Physiol. Cell Physiol. 292: C2239-C2258. Epub 2007 Feb 15.
2004 Lin, Y.F.*, Raab-Graham, K., Jan, Y.N. and Jan, L.Y. Nitric oxide stimulation of ATP-sensitive potassium channels: Involvement of Ras/MAPK kinase pathway and contribution to neuroprotection. Proc. Natl. Acad. Sci. USA 101: 7799-7804. (* as corresponding author)
2004 Bichet, D., Lin, Y.F., Ibarra, C.A., Huang, C.S., Yi, B.A., Jan, Y.N. and Jan, L.Y. Evolving potassium channels via yeast selection reveals structural elements important for selectivity. Proc. Natl. Acad. Sci. USA 101: 4441-4446.
2001 Yi, B.A., Minor, D.L., Lin, Y.F., Jan, Y.N. and Jan, L.Y. Controlling potassium channel activities: interplay between the membrane and intracellular factors. Proc. Natl. Acad. Sci. USA 98: 11016-11023.
2001 Yi, B.A., Lin, Y.F., Jan, Y.N. and Jan, L.Y. Yeast screen for constitutively active mutant G protein-activated potassium channels. Neuron 29: 657-667.
2001 Ma, D., Zerangue, N., Lin. Y.F., Collins, A., Yu, M., Jan, Y.N. and Jan, L.Y. Role of ER export signals in controlling surface potassium channel numbers. Science 291: 316-319.
2000 Minor, D.L., Lin, Y.F., Mobley, B.C., Avelar, A., Jan, Y.N., Jan, L.Y. and Berger, J.M. The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Cell 102: 657-670.
2000 Lin, Y.F., Jan, Y.N. and Jan, L.Y. Regulation of ATP-sensitive potassium channel function by protein kinase A-mediated phosphorylation in transfected HEK293 cell. EMBO J. 19: 942-955.
1996 Lin, Y.F., Angelotti, T.P., Dudek, E.M., Browning, M.D. and Macdonald, R.L. Protein kinase C phosphorylation of the Alpha 1 and Beta 2 gamma subunits of the GABAA receptor enhances whole-cell currents expressed in L929 fibroblasts. Mol. Pharmacol. 50: 185-195.
1994 Lin, Y.F., Browning, M.D., Dudek, E.M. and Macdonald, R.L. Protein kinase C enhances bovine Alpha 1 Beta 1 Gamma 2L GABAA receptor whole-cell currents expressed in L929 fibroblasts. Neuron 13: 1421-1431.
1992 Chai, C.Y., Wu, W.C., Wang, S., Su, C.K., Lin, Y.F., Yen, C.T., Kuo, J.S. and Wayner, M.J. Coexistence of autonomic and somatic mechanisms in the pressor areas of medulla in cats. Brain Res. Bull. 29: 15-26.
1991 Yen, C.T., Hwang, J.C., Su, C.K., Lin, Y.F., Yang, J.M. and Chai, C.Y. Differential actions of the median region of caudal medulla on autonomic nerve activities. Clin. Exp. Pharmacol. Physiol. 18: 743-751.
1990 Chai, C.Y., Lin, Y.F., Wang, H.Y., Wu, W.C., Yen, C.T., Kuo, J.S. and Wayner, M.J. Inhibition of spinal reflexes by paramedian reticular nucleus. Brain Res. Bull. 25: 581-588.
- Lothman Fellowship
- University of California Davis, Health System Research Award
- Harrison Endowed Chair in Diabetes Research Award
- American Heart Association Scientist Development Award (National Center)
- University of California Davis, Research Insurance Grant to Promote Extramural Funding
- American Physiological Society Travel Award
- University of California Davis, Genome Center Proteomics Core Faculty Pilot Project
- MCP 210A: Advanced Physiology (graduate course)
- HPH 400: Medical Physiology
- PTX 202: Principles of Pharmacology and Toxicology--Cellular and Molecular Basis of Drug Action (graduate course)
- HPH493/SUR493: Special Study Module for M4 students: Care of Critically Ill Surgical
- Patient: Use of Physiological Principles
- American Heart Association, National Center
- UC Davis Academic Senate
- UC Davis Health System