FacultyYu-Fung Lin, Ph.D.
Assistant Professor Recent/Current American Heart UC Davis Academic UC Davis Health Graduate Group Affiliations Biochemistry and |
Research Interests Our long-term research goal is to understand at a molecular level how ion channels are modulated by novel mechanisms and how we may manipulate channel regulation to treat diseases and protect cells from ischemic insults. In particular, we are interested in the ATP-sensitive potassium (KATP) channel, a high-fidelity metabolic sensor which sets membrane excitability to match cellular energy demand. Defects of KATP channel functionality are associated with diseases or pathophysiological states such as hypertension, vasospasm, diabetes mellitus, congenital hyperinsulinism, seizure, dilated cardiomyopathy, and ischemic/reperfusion injury. Our current work focuses on delineating the molecular mechanism and intracellular signaling pathway responsible for functional regulation of KATP channels by cyclic-nucleotide-dependent kinases and calcium. Other lines of research in my laboratory include studies of the role of KATP channels in ischemic preconditioning and regulation of GABA(A) receptors by novel messengers. Representative Publications Chai, C.Y., Lin, Y.F., Wang, H.Y., Wu, W.C., Yen, C.T., Kuo, J.S. and Wayner, M.J. Inhibition of spinal reflexes by paramedian reticular nucleus. Brain Res. Bull. 25: 581-588, 1990.Yen, C.T., Hwang, J.C., Su, C.K., Lin, Y.F., Yang, J.M. and Chai, C.Y. Differential actions of the median region of caudal medulla on autonomic nerve activities. Clin. Exp. Pharmacol. Physiol. 18: 743-751, 1991. Chai, C.Y., Wu, W.C., Wang, S., Su, C.K., Lin, Y.F., Yen, C.T., Kuo, J.S. and Wayner, M.J. Coexistence of autonomic and somatic mechanisms in the pressor areas of medulla in cats. Brain Res. Bull. 29: 15-26, 1992. Lin, Y.F., Browning, M.D., Dudek, E.M. and Macdonald, R.L. Protein kinase C enhances bovine Alpha 1 Beta 1 Gamma 2L GABAA receptor whole-cell currents expressed in L929 fibroblasts. Neuron 13: 1421-1431, 1994. Lin, Y.F., Angelotti, T.P., Dudek, E.M., Browning, M.D. and Macdonald, R.L. Protein kinase C phosphorylation of the Alpha 1 and Beta 2 gamma subunits of the GABAA receptor enhances whole-cell currents expressed in L929 fibroblasts. Mol. Pharmacol. 50: 185-195, 1996. Lin, Y.F., Jan, Y.N. and Jan, L.Y. Regulation of ATP-sensitive potassium channel function by protein kinase A-mediated phosphorylation in transfected HEK293 cell. EMBO J. 19: 942-955, 2000. Minor, D.L., Lin, Y.F., Mobley, B.C., Avelar, A., Jan, Y.N., Jan, L.Y. and Berger, J.M. The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Cell 102: 657-670, 2000. Ma, D., Zerangue, N., Lin. Y.F., Collins, A., Yu, M., Jan, Y.N. and Jan, L.Y. Role of ER export signals in controlling surface potassium channel numbers. Science 291: 316-319, 2001. Yi, B.A., Lin, Y.F., Jan, Y.N. and Jan, L.Y. Yeast screen for constitutively active mutant G protein-activated potassium channels. Neuron 29: 657-667, 2001. Yi, B.A., Minor, D.L., Lin, Y.F., Jan, Y.N. and Jan, L.Y. Controlling potassium channel activities: interplay between the membrane and intracellular factors. Proc. Natl. Acad. Sci. USA 98: 11016-11023, 2001. Bichet, D., Lin, Y.F., Ibarra, C.A., Huang, C.S., Yi, B.A., Jan, Y.N. and Jan, L.Y. Evolving potassium channels via yeast selection reveals structural elements important for selectivity. Proc. Natl. Acad. Sci. USA 101: 4441-4446, 2004. Lin, Y.F.*, Raab-Graham, K., Jan, Y.N. and Jan, L.Y. Nitric oxide stimulation of ATP-sensitive potassium channels: Involvement of Ras/MAPK kinase pathway and contribution to neuroprotection. Proc. Natl. Acad. Sci. USA 101: 7799-7804, 2004. (* as corresponding author) Mao, X., Chai, Y. and Lin, Y.F. Dual regulation of ATP-sensitive potassium channels by caffeine. Am. J. Physiol. Cell Physiol. 292: C2239-C2258, 2007. Epub 2007 Feb 15. Misaki, N., Mao, X., Lin, Y.F., Suga, S., Li, G.H., Liu, Q., Chang, Y., Wang, H, Wakui, M. and Wu, J. Iptakalim, a vascular KATP channel opener, closes rat pancreatic beta-cell KATP channels and increases insulin release. J. Pharmacol. Exp. Ther. 322: 871-878, 2007. Epub 2007 May 23. Lin, Y.F. and Chai, Y. Modulation of the ATP-sensitive potassium channel by extracellular signal-regulated kinase-mediated phosphorylation. Neuroscience 152: 371-380, 2008. Epub 2008 Jan 9. Chai, Y. and Lin, Y.F. Dual regulation of the ATP-sensitive potassium channel by activation of cyclic GMP-dependent protein kinase. Pflügers Arch.-European J. Physiol. 456: 897-915, 2008. Epub 2008 Jan 30. Recent/Current Teaching
Teaching and Research Awards
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