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Department of Pharmacology

Department of Pharmacology

Manuel Navedo, Ph.D.

Manuel Navedo, Ph.D.

Assistant Professor
Ph.D., University of Puerto Rico,, San Juan
(530) 752-6880

Research Interests

Cellular and molecular mechanisms regulating arterial smooth muscle excitability.

Research Synopsis

The main goal of my research team is to understand the mechanisms by which local and global calcium signals regulate excitation-contraction coupling and excitation-transcription coupling in arterial smooth muscle cells during physiological and pathological conditions.  To tackle these issues, we employ several state-of-the-art techniques including molecular biology, patch-clamp electrophysiology, and confocal and Total Internal Reflections Fluorescence (TIRF) microscopy.  We are currently interested in determining the mechanisms by which hyperglycemia induces the activation of a novel calcium signaling modality(persistent calcium sparklets) in arterial smooth muscle, causing increased contraction and consequently, arterial dysfunction during diabetes.

Selected Publications

LF Santana, MF Navedo (2009) Molecular and biophysical mechanisms of Ca2+ sparklets in smooth muscle. Journal of Molecular and Cellular Cardiology 47: 436-444.

MF Navedo*, T Takeda, M Nieves-Cintron, JD Molkentin, LF Santana (2010) Elevated calcium sparklet activity during acute hyperglycemia and diabetes in cerebral arterial smooth muscle cells. American Journal of Physiology 298: C211-C220. *corresponding author. This article is accompanied by an editorial from Dr. Mark T. Nelson highlighting the novel aspects of the study.

MF Navedo, EP Cheng, C Yuan, VS Votaw, JD Scott, LF Santana (2010) Increased coupled gating of L-type calcium channels during hypertension and Timothy syndrome. Circulation Research 106: 748-756. This article is accompanied by an editorial from Dr. Don Bers highlighting the novel aspects of the study.

LF Santana, MF Navedo (2010) Natural inequalities: why some L-type Ca2+ channels work harder than others. Journal of General Physiology 136: 143-147. Featured on the cover of the Journal.

EP Cheng, C Yuan, MF Navedo, RE Dixon, M Nieves-Cintrón, JD Scott, LF Santana. (2011) Restoration of normal L-type Ca2+ channel function during timothy syndrome by ablation of an anchoring protein. Circulation Research 109: 255-261.

Takeda, MA Nystoriak, M Nieves-Cintrón, LF Santana, MF Navedo* (2011) Relationship between calcium sparklets and sarcoplasmic reticulum calcium load and release in rat cerebral arterial smooth muscle. American Journal of Physiology - Heart 301: H2285-2294. *corresponding author.

RE Dixon, C Yuan, EP Cheng, MF Navedo, and LF Santana (2012) Calcium signaling amplification by oligomerization of L-type Cav1.2 channels. Proceedings of the National Academy of Science 109: 1749-1754.

Honors and Awards

  • 1996 University of Puerto Rico Dean's List
  • 2000 National Institute of Neurological Disorder and Stroke - Society for the Advancement of Chicanos and Native Americans in Science Neuroscience Fellowship
  • 2001 National Institute of Health Predoctoral Fellowship - Minority Biomedical Research Support (5R25GM61151)
  • 2004 National Institute of Health Postdoctoral Fellowship - Cardiovascular Training Program (T32-HL07828)