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Department of Pathology and Laboratory Medicine

Department of Pathology and Laboratory Medicine

Flow Cytometry and Immunology

Ranald Aitkens, C.L.S. (Supervisor)


The Immunology laboratory performs a variety of tests utilizing several methodologies. The specimen requirement for these tests is serum (red top tube or SST). While the menu is continually expanding the tests currently available are as follows:

Electrophoresis
  • Protein Electrophoresis (PEP)
  • Immunofixation (IFE)
Nephelometry
  • Immunoglobulin G (IGG)
  • Immunoglobulin A (IGA)
  • Immunoglobulin M (IGM)
  • Complement, C3
  • Complement, C4
  • Haptoglobin
  • Ceruloplasmin
  • Rheumatoid Factor (RF)
  • Pre-Albumin
Immunofluorescence
  • Anti-Nuclear Antibody (ANA)
  • Anti-dsDNA (DNA)
  • Anti-Neutrophil Cytoplasmic Antibodies (ANCA)
  • Anti-Parietal Cell Antibodies
  • Anti-Smooth Muscle Antibodies
  • Anti-Mitochrondial Antibodies
Agglutination/Flocculation
  • Syphilis Serology, RPR
  • Syphilis Serology, TPPA
    (Confirmatory test for Reactive RPR)
  • Syphilis Serology, VDRL (CSF)
Fluorescent Enzyme Immunoassay (FEIA)
  • Allergy Testing
  • IgE
Western Blot

Confirmatory test for specimens found to repeatedly reactive for HIV-1 using a screening procedure, such as Enzyme-linked Immunosorbent Assay (EIA)


Flow cytometry is included in the Immunology section of the lab. The Coulter FC500 Flow Cytometer offers state of the art cell surface marker recognition. Numerous surface markers as well as intracellular stains are offered in combinations intended to optimally define leukemic or lymphoid populations. Peripheral blood, bone marrow, fluid, or tissue samples are processed, stained then analyzed on the flow cytometer. Results are submitted for pathologist review and interpretation. Additionally, an Immunodeficiency panel is offered which includes CD4(T-cell helper), CD8(T-cell suppressor), CD3(total T-cell), CD4/CD8 ratio, absolute CD4 and absolute CD8. A CBC is required. When indicated, the pathologist may order additional markers or stains in order to clarify or support existing data.


The Immunology lab is staffed 7 days a week, day shift only.