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Flow Cytometry and Immunology

Kim Janatpour, M.D.  (Director)
Ranald Aitkens, C.L.S. (Supervisor)


The Immunology laboratory performs a variety of tests utilizing several methodologies. While the menu is continually expanding the tests currently available are as follows:

Electrophoresis Protein Electrophoresis (PEP)
Immunofixation (IFE)
Nephelometry Immunoglobulin G (IGG)
Immunoglobulin A (IGA)

Immunoglobulin M (IGM)

Complement, C3

Complement, C4

Haptoglobin

hs CRP

Ceruloplasmin

Rheumatoid Factor (RF)

Pre-Albumin

Micro-Albumin
Immunofluorescence Anti-Nuclear Antibody (ANA)
Anti-dsDNA (DNA)

Anti-Parietal Cell Antibodies

Anti-Smooth Muscle Antibodies

Anti-Mitochrondial Antibodies
Agglutination/Flocculation
Syphilis Serology, RPR

Syphilis Serology, TPPA

? (Confirmatory)

Syphilis Serology, VDRL (CSF)
Fluorescent Enzyme Immunoassay (FEIA)
Allergy Testing

IgE

Flow cytometry is included in the Immunology section of the lab. The Coulter Epics XL-MCL offers state of the art cell surface marker recognition. Numerous surface markers as well as intracellular stains are offered in combinations intended to optimally define leukemic or lymphoid populations. Peripheral blood, bone marrow, fluid, or tissue samples are processed, stained then analyzed on the flow cytometer. Results are submitted for pathologist review and interpretation. Additionally, an Immunodeficiency panel is offered which includes CD4(T-cell helper), CD8(T-cell suppressor), CD3(total T-cell), CD4/CD8 ratio, absolute CD4 and absolute CD8. A CBC is required. When indicated, the pathologist may order additional markers or stains in order to clarify or support existing data.


The Immunology lab is staffed 7 days a week, day shift only.