Flow Cytometry and Immunology
Katie Zegarski, MLS (ASCP) (Supervisor)
Flow Cytometry is an ancillary test that aides in the diagnosis of leukemia and lymphoma. Numerous surface markers, as well as, intracellular markers are offered in combination intended to optimally define leukemic or lymphoid populations. We offer 10-color flow cytometry on peripheral blood, bone marrow, fluid and tissue samples; which are processed, stained and analyzed in the flow cytometry lab. Results are submitted for hematopathologist review and interpretation. The typical turn-around time for our diagnostic flow cytometry results is within 1-3 days. Please also note that MRD (Minimal Residual Disease) panels are not available at this time in our flow cytometry laboratory. In addition to 10-color flow, we also perform Immunodeficiency Panels, used to monitor patients diagnosed with, and treated for, HIV; TBNK's, a basic immune status profile used to determine the % and absolute numbers of T-cells, B-cells and Natural Killer cells; CD3+T-cell levels, used to monitor transplant patients receiving anti-CD3 therapy; and CD19+B-cells, used to monitor B-cell levels in patients receiving anti-CD20 therapy. Please refer to the Laboratory Test Directory online for a comprehensive list of tests offered. The Flow Cytometry Lab is staffed 7 days a week, day shift only.
The Immunology Laboratory offers a variety of tests using different methodologies. Protein Electrophoresis is performed using a semi-automated, multi-parameter instrument. This high resolution procedure separates normal serum into six major fractions, which is used to screen serum and urine for protein abnormalities. Additionally, Immunofixation Electrophoresis is performed on serum and urine and is designed to detect monoclonal proteins presence. RAST allergy testing, which includes total and specific IGE, is performed on an automated instrument that measures the circulating antibody concentration in serum. The Immunology lab also performs an Indirect Immunofluorescence Assay to detect autoantibodies to Mitochondria, Smooth Muscle and Parietal Cells. This test aids in the diagnosis of autoimmune diseases. An alphabetic and detailed list of tests performed can be found online in our Laboratory Test Directory. Please contact the Immunology Laboratory for help with additional questions. The Immunology Lab is staffed 7 days a week, day shift only.