CoresLab Services
Contact Haynes Sheppard at hsheppar@dhs.ca.gov or (510) 307-8538 to discuss how the Virology and Immunology Core may be able to assist and/or collaborate with you in your AIDS research effort.
Specimen Processing
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Serum or plasma processing and storage: $15
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PBMC processing and storage: $35
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Specimen retrieval: $5
Cellular Immunology Assays
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Cytotoxic T-Lymphocyte (CTL) response (51Cr-release): $500
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ELISPOT assay (per plate): $150
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Intracellular cytokine and chemokine analysis: $100
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Proliferation (3H thymidine uptake; user supplied antigen): $50
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CD8-mediated virus suppression: $150
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Co-receptors (genetic polymorphism): $50
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Co-receptors (expression on lymphocytes): $75
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T lymphocyte immunophenotype (TLI) by flow cytometry
Standard panel (3 markers): $50 -
Extended panel: $75
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SCID-hu mice: negotiated
Humoral Immunity Assays
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Diagnostic EIA: $15
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Diagnostic western blot: $25
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Detuned serology: $20
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Synthetic peptide EIAs: $15/peptide
Antibody Neutralization
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MT-2 microplaque neutralizing antibody assay: $140
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Neutralization assays in primary cell cultures: $250
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Combination fusion inhibition/neutralization assay: $300
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Neutralization assays in tonsil histoculture: $300
Virologic Assays
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p24 antigen capture assay: $25
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Diagnostic DNA PCR (qualitative): $90
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Viral load RNA PCR (quantitative): $115
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NucliSense (ACTG certified laboratory): $115
Virus Isolation
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Individual tube culture (high sensitivity): $250
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Multi-well mini-cultures (good sensitivity): $100
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Quantitation of infectious units of HIV in PBMC and plasma: $250
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Virus expansion (100 mL): $150
Virus Characterization
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Nucleic acid purification and amplification: $50
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HMA/HTA: $100
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HIV DNA Sequencing
- - env: $200
- - gag: $200
- - pol: $200
- - ART resistance testing (Visible Genetics or ABI): $500
- - env: $200
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MT-2 tropism (SI/NSI): $50
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Co-receptor usage of HIV isolates: $100
Other
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Specialized or modified assays to be arranged negotiated: negotiated