IDDRC Biological Analysis Core
The overall objective of the Biological Analysis Core (BAC) is to facilitate an integrated experimental approach to the study of the molecular and cellular mechanisms of neuronal and immune function, including interactions between the two systems, in the context of IDD. The BAC is designed to advance and support research on emerging concepts regarding cellular and molecular mechanisms contributing to the risk and clinical heterogeneity of conditions associated with IDD, including variable response to therapy. Services provided will facilitate analyses of neuronal development, direct and indirect interactions between the neuronal and immune systems, cellular and molecular biology, and environmental exposures.
This state-of-the-art core provides users with four tiers of service: 1) consultation in model selection and experimental design; 2) training of users on available equipment; 3) training and supervision in conducting assays of interest where applicable; 4) battery of assays conducted by core staff; 5) tailored sets of analyses conducted by core staff. Combinations of 1-4 can be designed to match user needs. Assistance with data interpretation will be provided as needed for each tier.
- Cellular and Molecular Biology and Imaging. This subcore provides training and assistance with experimental design and data interpretation in cellular and molecular neurobiology and imaging techniques including: (1) primary culture of central and peripheral neurons derived from rodent models, including transgenic mice and rats; (2) immunocytochemical analyses of neural cell cultures; (3) Biolistic and Diolistic labeling of cells in dissociated neural cell cultures or brain slice cultures; (4) methods for expressing cDNA in cultured neurons; (5) morphometric analyses; (6) confocal microscopy; and (7) high content imaging.
Immunology. The Immunology subcore offers services in all general areas of immunologic analysis including, but not limited to: (1) Western blot analysis; (2) ELISA (e.g. immunoglobulins, antibody responses to specific antigens); (3) Luminex multiplex analysis (e.g. cytokines, chemokines, growth factors, neurotrophins); (4) cellular immunology including cell phenotyping using flow cytometry, as well as functional analysis, and immune function following targeted exposures. We are also able to design and perform immunohistochemical staining of tissue sections.
For questions, please contact Lori Haapanen, Project Manager firstname.lastname@example.org
Judy Van de Water, Ph.D., Core Director
Pamela Lein, Ph.D., Co-Director,
Paul Ashwood, Ph.D., Core Co-Manager
Ana Cristina Grodzki, BS, MS, Ph.D., Project Scientist
Lori Haapanen, Project Manager