Affymetrix GeneChip Analysis System
For standard RNA labeling investigators are required to submit 100-500 nanograms of high quality total RNA with 260/280 readings from a spectrophotometer and gel images of each sample submitted. Users must also provide the appropriate Affymetrix GeneChips.
For hybridizations only, investigators are required to provide 15 ug of high quality, labeled, and fragmented cRNA and the appropriate Affymetrix GeneChips. We also require gel images of each sample submitted, showing the initial RNA extraction and both the unfragmented and fragmented cRNA preps. Copies of the sample preparation protocols are available from the Affymetrix website.