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Department of Dermatology

Department of Dermatology

Dr. Isseroff Research Lab

Rivkah Isseroff, M.D.

Professor

Dr Isseroff

Research interests: Keratinocyte differentiation, migration and
wound healing, signal transduction, bioengineered tissue
replacement, and toxicology of the skin.

Dermatology Research
TB 192, UC Davis
One Shields Avenue, Davis, CA 95616
Email: rrisseroff@ucdavis.edu

Biography

Roslyn (Rivkah) Isseroff, M.D., is a Professor of Dermatology at UC Davis and Chief of the Dermatology Service VA Northern California Healthcare System, where she also heads the Wound Healing clinic. In her time at the University of California Davis, Dr. Isseroff has been a member or chair of multiple University-related committees as well as several professional societies such as the Society of Investigative Dermatology, American Dermatology Association, American Society for Cell Biology, and NIH Study section. She is also currently an associate editor for the Journal of Investigative Dermatology. Dr. Isseroff's honors and awards include the NIH Research Career Development Award, the UC Davis Dean's Award for Mentoring, grants from the NIH, CIRM, the Shriners Hospitals and the VA, and a number of patents. Her research interests focus on tissue repair and regeneration and how to translate these findings to new approaches to improve healing in patients who have chronic, non-healing wounds.

Lab Members

Dr Isseroff Research Lab Team 

 

Dr Isseroff Rivkah Isseroff, M.D.
Professor, Principal Investigator
E-mail: rrisseroff@ucdavis.edu

 

Dr Gorouhi Farzam Gorouhi, M.D.
CIRM Clinical Research Fellow
E-mail: fgorouhi@ucdavis.edu
Research Interests: Mesenchymal Stem Cell Therapy and Immunomodulation in Wound Healing

 

Dr Lev-tov Hadar Lev-Tov, M.D.
Clinical Research Fellow
E-mail: lhadar1@gmail.com
Research Interests: wound healing and outcomes research

 

Dr Yang Hsin-ya Yang, Ph.D.
Assistant Project Scientist
E-mail: hyang@ucdavis.edu
Education: National Taiwan University, B.S.
National Taiwan University, M.S.
UC Davis, Ph.D.
Research Interests: Directional migration of keratinocytes in an applied DC field and wound healing process

 

Dr Jagdeo Jared Jagdeo, M.D., M.S.
Assistant Professor, Director Photobiomodulation Research Group
E-mail: jrjagdeo@gmail.com
Education: Oxford University 2000-2001, Brown University 2002, Brown Graduate School 2006, Brown Medical School 2007, SUNY Downstate Medical Center, Dermatology Residency 2011, Howard Hughes Medical Institute – National Institute of Health (NIH) 2005-2006
Research Interests: Photomodulation of skin, light therapy for treatment of skin disorders, skin fibrosis

 

Marianne Chigbrow Marianne Chigbrow
Laboratory Manager
E-mail: mchigbrow@ucdavis.edu

 

Dr Sivamani Raja Sivamani, M.D., M.S.
Dermatology Resident, Research Track
E-mail: rksivamani@ucdavis.edu
Research interest: Botanical extracts, Phytochemicals, Sebocyte biology

 

Rasiq Zachria Rasiq Zachria
Undergraduate Researcher
E-mail: rasqisackria@gmail.com

 

Ravi Dasu

Ravi Dasu
Associate Researcher-Step I
E-mail: ravi.dasu@ucdmc.ucdavis.edu
Education: PhD in Zoology with Insect Biochemistry and Molecular biology as majors from Jiwaji University, India.
Other positions:  Adjunct Faculty, School of graduate studies, Drexel University, Sacramento Campus (Biochemistry, Immunology, Pathology) Adjunct Faculty, Dept of Biological Sciences, California State University, Sacramento (Molecular Biology)
Research Interests: Chronic wounds, Innate immunity, Diabetes complications, Inflammation, and Cell signaling.

Sandra Ramirez Sandra Ramirez
Junior Specialist
E-mail: srramirez@ucdavis.edu
Education: Animal Science, B.S. UC Davis
Research Interest:Mesenchymal Stem Cells, Innate immunity (TLR's)  and stress (beta adrenergic receptors), type 2 diabetic mouse models
  Shirley Vu
Junior Specialist
E-mail: smvu@ucdavis.edu

 

  Tina La
Junior Specialist
E-mail: tdinhla@udavis.edu

 

Visiting Researchers

Dr Peavy

Thomas Peavy, Ph.D.
Associate Professor of Biological Sciences, California State University, Sacramento
E-mail: trpeavy@csus.edu

 

Dr. Thomas Peavy's Website

Medical Students

Fakhra Khalid Fakhra Khalid
E-mail: fakhraikhalid@gmail.com

 

Andrew Mamalis Andrew Mamalis
E-mail: andrewmamalis@gmail.com

 

  Jennifer Ornelas
E-mail: jennifer.n.ornelas@gmail.com

 

  Lawrence Younan
E-mail: ljyounan@gmail.com

 

Graduate Students

Chuong Nguyen Chuong Nguyen
Ph.D. Candidate, Pharmacology/Toxiocology
E-mail: cjnguyen@ucdavis.edu
Research Interests: Mesenchymal stem cells, toxicology of adrenergic receptors, fat, aging, burns and diabetic wounds

Undergraduate Students

Brittany Gholson Brittany Gholson
E-mail: bgholson@ucdavis.edu

Past Lab Members

Dr Pullar Christine Pullar, Ph.D.
Current Position: Senior Lecturer, University of Leicester, Department of Cell Physiology and Pharmacology

 

Dr Kim Min-ho Kim, Ph.D.
Current Position: Assistant Professor, Bioengineering, Kent State University

 

Pieter Steenhuis Pieter Steenhuis
Current Position: Researcher, University Medical Center Hamburg-Eppendorf

 

Dr Wei Liu Wei Liu, M.D., Ph.D.
Current Position: Postdoctoral Fellow, Gurtner Lab, Stanford University

 

 

Research

Isseroff Lab - Skin Wound Healing 

Dr. Isseroff’s research interests include keratinocyte differentiation, migration and wound healing, signal transduction, bioengineered tissue replacement, and toxicology of the skin. Current research projects focus on analysis of cell responses to an applied electric field, bioengineered skin replacements, and enhancers of wound healing. She also directs a multi-specialty wound healing clinic within the VA Northern California Health Care System’s Sacramento Medical Center, which houses a clinical research unit actively carrying out both investigator and industry-initiated clinical trials for novel approaches to improve wound healing.

Publications

  1. Lev-Tov, H., Li, C., Dahle, S., Isseroff RR. (2013). Cellular versus acellular matrix devices in treatment of diabetic foot ulcers: study protocol for a comparative efficacy randomized controlled trial. Trials. 14(8).
  2. Dasu MR, Rivkah Isseroff R. (2012). Toll-like receptors in wound healing: location, accessibility, and timing. J Invest Dermatol. 132(8):1955-8. PubMed PMID: 22797299
  3. Isseroff RR, Dahle S. Electrical Stimulation Therapy and Wound Healing: Where Are We Now? Adv Wound Care, Mar 2012, epub ahead of print.
  4. Chahal DS, Sivamani RK, Rivkah Isseroff R, Dasu MR. Plant-Based Modulation of Toll-like Receptors: An Emerging Therapeutic Model. Phytother Res. 2012 Nov 12. Epub ahead of print
  5. Rizzo, A.E., Beckett, L.A., Baier, B.S., and Isseroff, R.R. (2012). The linear excisional wound: an improved model for human ex vivo wound epithelialization studies. Skin Res Technol 18, 125-132.
  6. Pullar, C.E., Le Provost, G.S., O'Leary, A.P., Evans, S.E., Baier, B.S., and Isseroff, R.R. (2012). beta2AR Antagonists and beta2AR Gene Deletion Both Promote Skin Wound Repair Processes. J Invest Dermatol 132, 2076-2084.
  7. Liu, W., Hsu, D.K., Chen, H.Y., Yang, R.Y., Carraway, K.L., 3rd: , Isseroff, R.R., and Liu, F.T. (2012). Galectin-3 Regulates Intracellular Trafficking of EGFR through Alix and Promotes Keratinocyte Migration. J Invest Dermatol.
  8. Dasu, M.R., Ramirez, S., and Isseroff, R.R. (2012). Toll-like receptors and diabetes: a therapeutic perspective. Clin Sci (Lond) 122, 203-214.
  9. Steenhuis, P., Huntley, R.E., Gurenko, Z., Yin, L., Dale, B.A., Fazel, N., and Isseroff, R.R. (2011). Adrenergic signaling in human oral keratinocytes and wound repair. J Dent Res 90, 186-192.
  10. Sivamani, R.K., Schwartz, M.P., Anseth, K.S., and Isseroff, R.R. (2011). Keratinocyte proximity and contact can play a significant role in determining mesenchymal stem cell fate in human tissue. FASEB J 25, 122-131.
  11. Liu, Y.C., Margolis, D.J., and Isseroff, R.R. (2011). Does inflammation have a role in the pathogenesis of venous ulcers? A critical review of the evidence. J Invest Dermatol 131, 818-827.
  12. Fitzmaurice, S.D., Sivamani, R.K., and Isseroff, R.R. (2011). Antioxidant therapies for wound healing: a clinical guide to currently commercially available products. Skin Pharmacol Physiol 24, 113-126.
  13. Zhao, M., Penninger, J., and Isseroff, R.R. (2010). Electrical Activation of Wound-Healing Pathways. Adv Skin Wound Care 1, 567-573.
  14. Lulevich, V., Yang, H.Y., Isseroff, R.R., and Liu, G.Y. (2010). Single cell mechanics of keratinocyte cells. Ultramicroscopy 110, 1435-1442.
  15. Isseroff, R.R., and Gomez, E.C. (2010). Vincent Azubike Ziboh (1929-2009). J Invest Dermatol 130, 1489-1490.
  16. Dasu, M.R., Thangappan, R.K., Bourgette, A., DiPietro, L.A., Isseroff, R., and Jialal, I. (2010). TLR2 expression and signaling-dependent inflammation impair wound healing in diabetic mice. Lab Invest 90, 1628-1636.
  17. Sivamani, R.K., Pullar, C.E., Manabat-Hidalgo, C.G., Rocke, D.M., Carlsen, R.C., Greenhalgh, D.G., and Isseroff, R.R. (2009). Stress-mediated increases in systemic and local epinephrine impair skin wound healing: potential new indication for beta blockers. PLoS Med 6, e12.
  18. Sivamani, R.K., Porter, S.M., and Isseroff, R.R. (2009). An epinephrine-dependent mechanism for the control of UV-induced pigmentation. J Invest Dermatol 129, 784-787.
  19. Lewkowitz-Shpuntoff, H.M., Wen, M.C., Singh, A., Brenner, N., Gambino, R., Pernodet, N., Isseroff, R., Rafailovich, M., and Sokolov, J. (2009). The effect of organo clay and adsorbed FeO(3) nanoparticles on cells cultured on Ethylene Vinyl Acetate substrates and fibers. Biomaterials 30, 8-18.
  20. Shi, B., Grahn, J.C., Reilly, D.A., Dizon, T.C., and Isseroff, R.R. (2008). Responses of the 27-kDa heat shock protein to UVB irradiation in human epidermal melanocytes. Exp Dermatol 17, 108-114.
  21. Pullar, C.E., Manabat-Hidalgo, C.G., Bolaji, R.S., and Isseroff, R.R. (2008). beta-Adrenergic receptor modulation of wound repair. Pharmacol Res 58, 158-164.
  22. Mori, S., Wu, C.Y., Yamaji, S., Saegusa, J., Shi, B., Ma, Z., Kuwabara, Y., Lam, K.S., Isseroff, R.R., Takada, Y.K., et al. (2008). Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. J Biol Chem 283, 18066-18075.
  23. Kurzrock, E.A., Lieu, D.K., Degraffenried, L.A., Chan, C.W., and Isseroff, R.R. (2008). Label-retaining cells of the bladder: candidate urothelial stem cells. Am J Physiol Renal Physiol 294, F1415-1421.
  24. Kim, M.H., Liu, W., Borjesson, D.L., Curry, F.R., Miller, L.S., Cheung, A.L., Liu, F.T., Isseroff, R.R., and Simon, S.I. (2008). Dynamics of neutrophil infiltration during cutaneous wound healing and infection using fluorescence imaging. J Invest Dermatol 128, 1812-1820.
  25. Ghoghawala, S.Y., Mannis, M.J., Pullar, C.E., Rosenblatt, M.I., and Isseroff, R.R. (2008). Beta2-adrenergic receptor signaling mediates corneal epithelial wound repair. Invest Ophthalmol Vis Sci 49, 1857-1863.
  26. Sivamani, R.K., Lam, S.T., and Isseroff, R.R. (2007). Beta adrenergic receptors in keratinocytes. Dermatol Clin 25, 643-653, x.
  27. Sivamani, R.K., Garcia, M.S., and Isseroff, R.R. (2007). Wound re-epithelialization: modulating keratinocyte migration in wound healing. Front Biosci 12, 2849-2868.
  28. Pullar, C.E., Zhao, M., Song, B., Pu, J., Reid, B., Ghoghawala, S., McCaig, C., and Isseroff, R.R. (2007). Beta-adrenergic receptor agonists delay while antagonists accelerate epithelial wound healing: evidence of an endogenous adrenergic network within the corneal epithelium. J Cell Physiol 211, 261-272.
  29. Nguyen, M.M., Lieu, D.K., deGraffenried, L.A., Isseroff, R.R., and Kurzrock, E.A. (2007). Urothelial progenitor cells: regional differences in the rat bladder. Cell Prolif 40, 157-165.
  30. Margolis, D.J., Hoffstad, O., and Isseroff, R.R. (2007). Association between the use of beta-adrenergic receptor agents and the development of venous leg ulcers. Arch Dermatol 143, 1275-1280.
  31. Lieu, D.K., Degraffenried, L.A., Isseroff, R.R., and Kurzrock, E.A. (2007). Beta1 integrin expression pattern in transitional urothelium does not allow for efficient stem cell enrichment as in other epithelia. Tissue Eng 13, 263-270.
  32. Ghoghawala, S.Y., Mannis, M.J., Murphy, C.J., Rosenblatt, M.I., and Isseroff, R.R. (2007). Economical LED based, real-time, in vivo imaging of murine corneal wound healing. Exp Eye Res 84, 1031-1038.
  33. Sun, Y., Frenkel, A.I., Isseroff, R., Shonbrun, C., Forman, M., Shin, K., Koga, T., White, H., Zhang, L., Zhu, Y., et al. (2006). Characterization of palladium nanoparticles by using X-ray reflectivity, EXAFS, and electron microscopy. Langmuir 22, 807-816.
  34. Shi, B., and Isseroff, R.R. (2006). Arsenite pre-conditioning reduces UVB-induced apoptosis in corneal epithelial cells through the anti-apoptotic activity of 27 kDa heat shock protein (HSP27). J Cell Physiol 206, 301-308.
  35. Shi, B., Han, B., Schwab, I.R., and Isseroff, R.R. (2006). UVB irradiation-induced changes in the 27-kd heat shock protein (HSP27) in human corneal epithelial cells. Cornea 25, 948-955.
  36. Pullar, C.E., Rizzo, A., and Isseroff, R.R. (2006). beta-Adrenergic receptor antagonists accelerate skin wound healing: evidence for a catecholamine synthesis network in the epidermis. J Biol Chem 281, 21225-21235.
  37. Pullar, C.E., and Isseroff, R.R. (2006). The beta 2-adrenergic receptor activates pro-migratory and pro-proliferative pathways in dermal fibroblasts via divergent mechanisms. J Cell Sci 119, 592-602.
  38. Pullar, C.E., Grahn, J.C., Liu, W., and Isseroff, R.R. (2006). Beta2-adrenergic receptor activation delays wound healing. FASEB J 20, 76-86.
  39. Pullar, C.E., Baier, B.S., Kariya, Y., Russell, A.J., Horst, B.A., Marinkovich, M.P., and Isseroff, R.R. (2006). beta4 integrin and epidermal growth factor coordinately regulate electric field-mediated directional migration via Rac1. Mol Biol Cell 17, 4925-4935.
  40. Mukerjee, E.V., Isseroff, R.R., Nuccitelli, R., Collins, S.D., and Smith, R.L. (2006). Microneedle array for measuring wound generated electric fields. Conf Proc IEEE Eng Med Biol Soc 1, 4326-4328.
  41. Stipcevic, T., Piljac, T., and Isseroff, R.R. (2005). Di-rhamnolipid from Pseudomonas aeruginosa displays differential effects on human keratinocyte and fibroblast cultures. J Dermatol Sci 40, 141-143.
  42. Shirakawa, M., and Isseroff, R.R. (2005). Topical negative pressure devices: use for enhancement of healing chronic wounds. Arch Dermatol 141, 1449-1453.
  43. Shi, B., and Isseroff, R.R. (2005). Epidermal growth factor (EGF)-mediated DNA-binding activity of AP-1 is attenuated in senescent human epidermal keratinocytes. Exp Dermatol 14, 519-527.
  44. Salehi-Had, H., Alvarenga, L.S., Isseroff, R., and Schwab, I.R. (2005). Factors modulating p63 expression in cultured limbal epithelial cells. Cornea 24, 845-852.
  45. Pullar, C.E., and Isseroff, R.R. (2005). Cyclic AMP mediates keratinocyte directional migration in an electric field. J Cell Sci 118, 2023-2034.
  46. Pullar, C.E., and Isseroff, R.R. (2005). Beta 2-adrenergic receptor activation delays dermal fibroblast-mediated contraction of collagen gels via a cAMP-dependent mechanism. Wound Repair Regen 13, 405-411.
  47. Kurzrock, E.A., Lieu, D.K., deGraffenried, L.A., and Isseroff, R.R. (2005). Rat urothelium: improved techniques for serial cultivation, expansion, freezing and reconstitution onto acellular matrix. J Urol 173, 281-285.
  48. Kang, S.H., Fung, M.A., Gandour-Edwards, R., Reilly, D., Dizon, T., Grahn, J., and Isseroff, R.R. (2004). Heat shock protein 27 is expressed in normal and malignant human melanocytes in vivo. J Cutan Pathol 31, 665-671.
  49. Ionides, E.L., Fang, K.S., Isseroff, R.R., and Oster, G.F. (2004). Stochastic models for cell motion and taxis. J Math Biol 48, 23-37.
  50. Grahn, J.C., and Isseroff, R.R. (2004). Human melanocytes do not express EGF receptors. J Invest Dermatol 123, 244-246.
  51. Sillman, A.L., Quang, D.M., Farboud, B., Fang, K.S., Nuccitelli, R., and Isseroff, R.R. (2003). Human dermal fibroblasts do not exhibit directional migration on collagen I in direct-current electric fields of physiological strength. Exp Dermatol 12, 396-402.
  52. Shi, B., Isseroff, R.R., and Nuccitelli, R. (2003). Power line frequency electromagnetic fields do not increase the rate of protein synthesis in human skin fibroblasts as previously reported. Bioelectromagnetics 24, 465-472.
  53. Shi, B., Farboud, B., Nuccitelli, R., and Isseroff, R.R. (2003). Power-line frequency electromagnetic fields do not induce changes in phosphorylation, localization, or expression of the 27-kilodalton heat shock protein in human keratinocytes. Environ Health Perspect 111, 281-288.
  54. Pullar, C.E., Chen, J., and Isseroff, R.R. (2003). PP2A activation by beta2-adrenergic receptor agonists: novel regulatory mechanism of keratinocyte migration. J Biol Chem 278, 22555-22562.
  55. Ojingwa, J.C., and Isseroff, R.R. (2003). Electrical stimulation of wound healing. J Invest Dermatol 121, 1-12.
  56. Grahn, J.C., Reilly, D.A., Nuccitelli, R.L., and Isseroff, R.R. (2003). Melanocytes do not migrate directionally in physiological DC electric fields. Wound Repair Regen 11, 64-70.
  57. Trollinger, D.R., Isseroff, R.R., and Nuccitelli, R. (2002). Calcium channel blockers inhibit galvanotaxis in human keratinocytes. J Cell Physiol 193, 1-9.
  58. Han, B., Schwab, I.R., Madsen, T.K., and Isseroff, R.R. (2002). A fibrin-based bioengineered ocular surface with human corneal epithelial stem cells. Cornea 21, 505-510.
  59. Chen, J., Hoffman, B.B., and Isseroff, R.R. (2002). Beta-adrenergic receptor activation inhibits keratinocyte migration via a cyclic adenosine monophosphate-independent mechanism. J Invest Dermatol 119, 1261-1268.
  60. Pullar, C.E., Isseroff, R.R., and Nuccitelli, R. (2001). Cyclic AMP-dependent protein kinase A plays a role in the directed migration of human keratinocytes in a DC electric field. Cell Motil Cytoskeleton 50, 207-217.
  61. deGraffenried, L.A., and Isseroff, R.R. (2001). Wound dressings alter the colony-forming efficiency of keratinocytes in cultured sheet grafts. Cell Transplant 10, 749-754.
  62. Wong, J.W., Shi, B., Farboud, B., McClaren, M., Shibamoto, T., Cross, C.E., and Isseroff, R.R. (2000). Ultraviolet B-mediated phosphorylation of the small heat shock protein HSP27 in human keratinocytes. J Invest Dermatol 115, 427-434.
  63. Shi, B., and Isseroff, R.R. (2000). Divergent responses of ras-transfected and non-ras-transfected human keratinocytes to extracellular calcium. Biochem Cell Biol 78, 469-476.
  64. Schwab, I.R., Reyes, M., and Isseroff, R.R. (2000). Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease(1). Am J Ophthalmol 130, 543-544.
  65. Schwab, I.R., and Isseroff, R.R. (2000). Bioengineered corneas--the promise and the challenge. N Engl J Med 343, 136-138.
  66. Farboud, B., Nuccitelli, R., Schwab, I.R., and Isseroff, R.R. (2000). DC electric fields induce rapid directional migration in cultured human corneal epithelial cells. Exp Eye Res 70, 667-673.
  67. Obedencio, G.P., Nuccitelli, R., and Isseroff, R.R. (1999). Involucrin-positive keratinocytes demonstrate decreased migration speed but sustained directional migration in a DC electric field. J Invest Dermatol 113, 851-855.
  68. Fang, K.S., Ionides, E., Oster, G., Nuccitelli, R., and Isseroff, R.R. (1999). Epidermal growth factor receptor relocalization and kinase activity are necessary for directional migration of keratinocytes in DC electric fields. J Cell Sci 112 ( Pt 12), 1967-1978.
  69. Fang, K.S., Farboud, B., Nuccitelli, R., and Isseroff, R.R. (1998). Migration of human keratinocytes in electric fields requires growth factors and extracellular calcium. J Invest Dermatol 111, 751-756.
  70. Shi, B., and Isseroff, R.R. (1997). Intracellular calcium oscillations in cell populations of ras-transfected I-7 subline of human HaCaT keratinocytes. J Invest Dermatol 109, 765-769.
  71. Shi, B., and Isseroff, R.R. (1996). Thapsigargin induces phosphorylation of the 27-kDa heat shock protein in human keratinocytes. J Invest Dermatol 107, 749-754.
  72. Sheridan, D.M., Isseroff, R.R., and Nuccitelli, R. (1996). Imposition of a physiologic DC electric field alters the migratory response of human keratinocytes on extracellular matrix molecules. J Invest Dermatol 106, 642-646.
  73. Nishimura, K.Y., Isseroff, R.R., and Nuccitelli, R. (1996). Human keratinocytes migrate to the negative pole in direct current electric fields comparable to those measured in mammalian wounds. J Cell Sci 109 ( Pt 1), 199-207.