- Operator observation and interaction.
- Parametric monitoring
- Chemical monitoring
- Biological monitoring
- Diagnostic cycle
Operator cbservation and interaction
At the end of the cycle, the sterilant cup is checked to note the complete aspiration of the active ingredient. If fluid is noted in the cup, appropriate disposal methods must be employed according to the manufacturer's instructions.
Biological monitoring consists of a test challenge compared to a control culture obtained from a live spore strip of Bacillus stearothermophilus. The frequency to the testing will be determined by the health care facility's internal policy. A chemical indicator should be run with all biological testing. After appropriate incubation, the test challenge is compared with the control and the results are recorded.
A failed diagnostic cycle will lock out any attempts to use the sterilizer until the fault has been corrected, and a successful diagnostic cycle is completed. The cup of sterilant is not used when running a diagnostic cycle.
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