Department of Cell Biology and Human Anatomy
John Hess, Ph.D.
The lens contains two distinct Intermediate Filament (IF) related cytoskeletal structures: vimentin composed IFs and filensin and phakinin composed Beaded Filaments. The detailed structure of both of these structures is not known.
In collaboration with John Voss, Dr. Hess has studied vimentin structure and assembly using site directed spin labeling and EPR. He has also performed experiments adapting the chaperone activity of alpha crystallin to maintain the solubility of proteins expressed in E. coli and in vitro in solution.
Finally, in collaboration with John Angelos, Dr. Hess has helped clone and characterize a hemolysin expressed by Moraxella bovis, an opportunistic ocular pathogen of cattle, and conducted studies using the hemolysin as a potential vaccine.
2006 Hess, JF and Angelos, JA. The Moraxella bovis RTX locus mbx defines a pathogenicity island. J. Med. Micro. 55(4) 443-9.
2006 Hess JF, Budamagunta MS, Shipman, R. L., Voss JC, FitzGerald PG, Characterization of the linker 2 region in human vimentin using site-directed spin labeling and electron paramagnetic resonance. Biochemistry 45 (39) p 11737-11743.
2007 Angelos JA, Bonifacio RG, Ball LM, Hess JF. Prevention of naturally occurring infectious bovine keratoconjunctivitis with a recombinant Moraxella bovis pilin- Moraxella bovis cytotoxin-ISCOM matrix adjuvanted vaccine.Vet Microbiol. 2007 Dec 15;125(3-4):274-83.