Director: Emmanual Maverakis, M.D.
The Immune Monitoring Shared Resource (IMSR) provides comprehensive, up-to-date immune monitoring services for clinical and translational studies to support investigators and industry partners who want to characterize the immune system in the setting of disease, especially autoimmunity and cancer. The IMSR provides the mechanistic assay infrastructure needed to support ancillary studies to clinical trials and facilitate biomarker and discovery research. The IMSR develops and validates immune monitoring assays, manages immune-relevant specimens including their tracking and storage, conducts on-site training for cancer center investigators and conducts quality assurance/quality control activities. The IMSR also facilitates collaborative and contracted arrangements with other academic facilities and industry partners.
It also maintains a centralized approved IRB for specimen collection and characterization for specimens collected from patients with any immune-mediated disease or immune-responsive cancer such as melanoma, lung and kidney.
In addition to immune monitoring, the IMSR also maintains a variety of protocols to study the human microbiome.
This resource is funded by the UC Davis Comprehensive Cancer Center Support Grant (CCSG) awarded by the National Cancer Institute (NCI P30CA093373). Publications that have utilized facility resources, services or scientific data generated by the resource should acknowledge the resource or the assistance provided by resource staff and cite the NCI CCSG grant. Moreover, to comply with NIH Public Access Policy, you must ensure that the manuscript is deposited in PubMedCentral. An electronic copy of the publication should also be sent to the resource directors.
- Basic Standardized Service - The IMSR offers immune cell profiling via flow cytometry including intracellular cytokine detection and cell surface phenotyping. It also offers multiplex analyte detection (Luminex-based multiplex assays), immune cellular assays (CFSE dilution, antigen-specific and non-specific responses and cytokine production), and gene expression profiling (Illumina-based whole transcriptome sequencing, qRT-PCR arrays, etc.). Keeping cost low is a priority for all the services provided.
- Banking Service - The IMSR offers patient specimen processing and storage using electronic sample management and tracking systems. Verified and validated standard SOPs are followed for sample acquisition, isolation, storage and distribution. Standard SOPs provide consistent processes for all research activities from sample to sample, investigator to investigator, and study to study. Peripheral blood is ideal for immune monitoring; however, the IMSR also has established protocols for sample processing obtained from other sources such as skin, tumor, saliva and urine. Large reliable data stores protect all information.
- Murine models of autoimmunity and cancer - The IMSR has experience in a variety of mouse models of autoimmune diseases such as alopecia areata, psoriasis, scleroderma, atopic dermatitis and collagen-induced arthritis. Cancer models include xenograft models and murine models of melanoma.
- Consulting Service - The IMSR provides individual consultation on experimental design and appropriate sample collection for analysis. We offer expert advice for optimal project-specific immunologic assessment and innovative approaches to evaluate immune responses in clinical studies.
- Bio-Plex 200 System capable of multiplexing up to 100 analytes simultaneously in a single well using only 25 ul of samples
- Tissue culture room for patient sample processing and to perform immune function assays, 3 laminar hoods, 4 tissue culture incubators, centrifuges (two Eppendorf 5425R and 5810R), microscope and water bath
- Infrastructure for sample collection, processing and storage with dedicated -20C and -80C freezers and two large Liquid nitrogen storage units with temperature monitors
- QiaCube robot for automated nucleic acid isolation
- Agilent 2200 TapeStation to check RNA/DNA/protein integrity
- Droplet Digital PCR (Bio-Rad)
- Tecan Infinite M1000 PRO microplate reader - The most state of the art plate reader on the market. It can do 3D imaging and top and bottom reads.
- Nikon Eclipse TS100 Fluorescent microscope and camera
- 3 PCR cabinet work stations
- Bio-Rad CFX96 quantitative real-time PCR System
- Five 96-well PCR machines
- Qiagen TissueLyser II
- Qubit Fluorometer
- Hollow fiber Bioreactor for monoclonal antibody production
- Three high-speed shakers (with thermo capabilities) Illumina
- MiSeq next generation sequencer