Yoshihiro Izumiya D.V.M. Ph.D. and his laboratory study epigenetic gene regulation by using herpesvirus reactivation as a model system. Many viruses produce an acute infection in the human host and are then cleared by the immune system. However, other viruses, especially herpesvirus, remain in the infected person till death by producing a chronic latent infection. In latent infection, the herpesvirus hides from the immune system by silencing their own gene expression and no infectious virus can be detected during latency. The virus can be reactivated with different external stimuli and will then activate hundreds of gene expression in cascade fashion; this provides us a compelling model to study movement and regulation from “silenced chromatin” to “active chromatin” of host genome.
Dr. Izumiya’s lab is particularly interested in the epigenetic components of chromatin, including host and viral factors that interact to facilitate both the establishment and maintainance of the chromatin state as well as the molecular transitions that take place during reactivation (“chromatin remodeling”). In addition, post-translational modifications that are imparted on critical viral and host factors are also an area of research in his lab. Using a combination of genetic and biochemical approaches, they try to understand the role of host and viral protein modifications such as phosphorylation, methylation, sumoylation and ubiquitinylation on protein function and role in the viral life cycle. Their research provides insights into both the post-translational modifications and chromatin remodeling that governs viral latency and reactivation. Moreover, their research may shed new information applicable to other areas of biology, such as cancer, development and aging, where epigenetic regulation has been implicated.
Pei-Ching Chang, Yoshihiro Izumiya, Chun-Yi Wu, Latricia D. Fitzgerald, Mel Campbell, Thomas J. Ellison, Kit S.Lam, Paul A. Luciw and Hsing-Jien Kung (2010) Kaposi's Sarcoma-associated Herpesvirus (KSHV) Encodes a SUMO E3 ligase That Is SIM-dependent and SUMO-2/3-specific. Journal of Biological Chemistry 285: 5266-5273
Yoshihiro Izumiya*, Chie Izumiya, Datsun Hsia, Thomas J. Ellison, Paul A. Luciw, and Hsing-Jien Kung (2009) NF-kB Serves as a Cellular Sensor of KSHV Latency and Negatively Regulates K-Rta by Antagonizing the RBP-Jk Coactivator. Journal of Virology 83: 4435-4446 * Corresponding author
Thomas J. Ellison#, Yoshihiro Izumiya#*, Chie Izumiya, Paul A. Luciw, and Hsing-Jien Kung (2009) A Comprehensive Analysis of Recruitment and Transactivation Potential of K-Rta and K-bZIP during Reactivation of Kaposi’s Sarcoma-Associated Herpesvirus. Virology 387: 76-88 * Corresponding author #share first authorship
Pei-Ching Chang, Latricia D. Fitzgerald, Albert Van Geelen, Yoshihiro Izumiya, Thomas J. Ellison, Don-Hong Wang, David K. Ann, Paul A. Luciw and Hsing-Jien Kung (2009) Kruppel-Associated Box Domain-Associated Protein-1 as a Latency Regulator for Kaposi's Sarcoma-Associated Herpesvirus and Its Modulation by the Viral Protein Kinase. Cancer Research 69: 5681-5689.
Paulette F. Suchodolski#, Yoshihiro Izumiya#, Blanca Lupiani, Dharani K. Ajithdoss, Oren Gilad, Lucy F. Lee, Hsing-Jien Kung, and Sanjay M. Reddy (2009) Homodimerization of Marek's Disease Virus-Encoded Meq Protein Is Not Sufficient for Transformation of Lymphocytes in Chickens. Journal of Virology 83: 859-869 #share first authorship
Oliver Gautschi, Clifford G. Tepper, Phillip R. Purnell, Yoshihiro Izumiya, Christopher P. Evans, Tim P. Green, Pierre Y. Desprez, Primo N. Lara, David R. Gandara, Philip C. Mack, and Hsing-Jien Kung (2008) Regulation of Id1 Expression by Src: Implications for Targeting of the Bone Morphogenetic Protein Pathway in Cancer. Cancer Research 68: 2250-2258
Yoshihiro Izumiya, Chie Izumiya, Albert M. VanGeelen, Don-Hong Wang, Kit S. Lam, Paul A. Luciw, Hsing-Jien Kung (2007) Kaposi's sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP. Journal of Virology 81: 1072-1082
Alon M. Levy, Oren Gilad, Liang Xia, Yoshihiro Izumiya, Jonathan Choi, Tsalenko A, Yakhini Z, Richard L. Witter, Lucy F. Lee, Carol J. Cardona, Hsing-Jien Kung. (2005) Meq transforms chicken cells via the v-Jun transcriptional cascade: a converging transforming pathway for Avian oncoviruses. Proceedings National Academy of Science 102:14831-14836
Yoshihiro Izumiya, Thomas J. Ellison, Edward T.H. Yeh, Jae U. Jung, Paul A. Luciw, Hsing-Jien Kung. (2005) Kaposi’s sarcoma-associated Herpesvirus K-bZIP represses gene transcription via SUMO modification. Journal of Virology 79:9912-9925