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Department of Biochemistry and Molecular Medicine

Department of Biochemistry and Molecular Medicine

Wenbin Deng, Ph.D.

Wenbin Deng, Ph.D.

Associate Professor
653B Shriner's Hospital
Sacramento Campus
Ph: (916) 453-2287
e-mail

Research Interests

The Deng Lab is interested in studying neural development and disease. We investigate how stem cells differentiate and give rise to neurons and glia, and then the grey matter and white matter and connected regions, and how variations in basic programs of development underlie nervous system disorders. We use mouse and human model systems, genetic fate mapping strategies, cellular and behavioral phenotyping, and high throughput library or omics techniques. Current projects focus on the regulation of neural stem cell identity and differentiation using human induced pluripotent stem cells (hiPSC) differentiated into neurons and glia and their interconnected networks in vitro, genetic and epigenetic regulation of neural development and disease in hiPSC models or using mouse models, and stem cell responses to environmental perturbations, including hypoxia-ischemia, oxidative stress, and inflammation. We then use these models and technology platforms to identify therapeutic targets and intervention strategies.

 

Representative Publications

Jiang P, Chen C, Wang R, Chechneva OV, Chung S, Rao M, Pleasure DE, Liu Y, Zhang Q, Deng W. (2013) hESC-derived Olig2+ progenitors generate a subtype of astrocytes with protective effects against ischemic brain injury. Nature Communications, 4:2196 doi: 10.1038/ncomms3196.

Daugherty DJ, Selvaraj S, Chechneva OV, Liu X, Pleasure DE, Deng W. (2013) A TSPO ligand is protective in a mouse model of multiple sclerosis. EMBO Mol Med, 5 (6): 891-903.

Jiang P, Chen C, Liu X, Selvaraj V, Liu W, Feldman DH, Liu Y, Pleasure DE, Li, RA, Deng W. (2013) Generation and characterization of spiking and nonspiking oligodendroglial precursor cells from embryonic stem cells. Stem Cells, 31 (9), doi: 10.1002/stem.1515.

Chung S, Guo F, Jiang P, Pleasure DE, Deng W. (2013) Olig2/Plp+ progenitor cells give rise to Bergmann glia in the cerebellum. Cell Death & Dis, 4:e546. doi:10.1038/cddis.2013.74. PMID: 23492777.

Liu X, Shen Y, Plane JM, Deng W. (2013) Vulnerability of premyelinating oligodendrocytes to white matter damage in neonatal brain injury. Neurosci Bulletin, 29 (2): 229–238. PubMed ID: 23456565.

Zhou B, Si W, Su Z, Deng W, Tu X, Wang Q. (2013) Transcriptional activation of the Prox1 gene by HIF-1α and HIF-2α in response to hypoxia. FEBS Lett 587 (6): 724-731.

Liu W, Deng Y, Liu Y, Gong W, Deng W. (2013) Stem cell models for drug discovery and toxicology studies. J Biochem Mol Toxicol, 27 (1): 17-27.

Sohn J, Selvaraj V, Wakayama K, Orosco L, Lee E, Crawford SE, Guo F, Lang J, Horiuchi M, Zarbalis K, Itoh T, Deng W, Pleasure DE. (2012) PEDF is a novel oligodendrogenic morphogen acting on the adult SVZ and corpus callosum. J Neurosci, 32 (35):12152-12164.

Plane JM, Grossenbache SK, Deng W. (2012) Poly-ADP-ribose polymerase-1 deletion switches mouse subventricular zone stem cells toward a glial fate. J Neurosci Res, 90 (8):1489-1506.

Shen Y, Liu X, Pleasure DE, Deng W. (2012) Axon-glia synapses are highly vulnerable to white matter injury in the developing brain. J Neurosci Res, 90 (1):105-121.

Calafiore M, Copani, A, Deng W. (2012) DNA polymerase-ß mediates the neurogenic effect of ß-amyloid protein on subventricular zone stem cells. J Neurosci Res, 90: 559-567.

Chen C, Daugherty DJ, Jiang P, Deng W. (2012) Oligodendrocyte progenitor cells derived from mouse embryonic stem cells give rise to type-1 and type-2 astrocytes in vitro. Neurosci Lett, 523 (2):180-185.

Selvaraj V, Jiang P, Chechneva O, Lo U, Deng W. (2012) Differentiating human stem cells into neurons and glial cells. Frontiers in Biosciences, 17: 65-89. The first two authors contributed equally to this work.

Liu Y, Jiang P, Deng W. (2011) Olig gene targeting in human pluripotent stem cells for motor neuron and oligodendrocyte differentiation. Nature Protocols, 6(5): 640-655. The first two authors contributed equally.

Chung S, Calafiore M, Plane JM, Pleasure D, Deng W. (2011) AIF deficiency causes reduced mitofusion-1 expression and patterned Purkinje cell degeneration. Neurobiology of Disease, 41: 445–457.

Liu W, Shen Y, Plane JM, Pleasure DE, Deng W. (2011) Neuroprotective potential of erythropoietin and its derivative carbamylated erythropoietin in periventricular leukomalacia. Experimental Neurology, 230: 227-239 (selected for COVER illustration).

Zhang Jiaping, Marco Calafiore, Yuesheng Huang, Qunli Zeng, Xiuzhen Zhang, Ronald A. Li, Deng W*, Min Zhao*. (2011) Electrically guiding migration of human induced pluripotent stem cells. Stem Cell Rev Reports, 7: 987-996. PubMed ID: 21373881. *Co-correspondence authors.

Lo K, Sun Y, Landry JP, Zhu X, Deng W. (2011) Label-free detection of surface markers on stem cells by oblique-incidence reflectivity difference microscopy. BioTechniques, 50 (6): 381–388.

Wang Y, Plane JM, Jiang P, Zhou C, Deng W. (2011) Quiescent and active stages of endogenous adult neural stem cells: identification and characterization. Stem Cells, 29 (6):907-912.

Soundarapandian MM, Selvaraj V, Lo U, Golub MS, Feldman DH, Pleasure DE, Deng W. (2011) Zfp488 promotes oligodendrocyte differentiation of neural progenitor cells in adult mice after cuprizone-induced demyelination. Sci. Reports, 1:2 doi:10.1038/srep2 (2011). PMID: 22355521.
http://www.nature.com/srep/2011/110614/srep00002/full/srep00002.html

Chechneva OV, Mayrhofer F, Daugherty DJ, Pleasure DE, Hong J, Deng W. (2011) Low dose dextromethorphan attenuates moderate experimental autoimmune encephalomyelitis by inhibiting NOX2 and reducing peripheral immune cells infiltration in the spinal cord. Neurobiology of Disease, 44: 63-72.

Jantzie LL, Talos DM, Selip DB, An L, Jackson MC, Folkerth RD, Deng W, Jensen FE (2011) Developmental regulation of group I metabotropic glutamate receptors in the premature brain and their protective role in a rodent model of periventricular leukomalacia. Neuron Glia Biology, 6 (4): 277-288. Epub 2011 Dec 14.

Calafiore M, Copani, A, Deng W. (2011) DNA polymerase-ß mediates the neurogenic effect of ß-amyloid protein on subventricular zone stem cells. J Neurosci Res, doi: 10.1002/jnr.22780.

Deng W. (2010) Neurobiology of injury to the developing brain. Nature Reviews Neurology, 6 (6): 328-336.

Liu D, Ander BP, Xu H, Shen Y, Kaur P, Deng W, Sharp FR. (2010) Blood brain barrier breakdown and repair by src after thrombin-induced brain injury. Annals of Neurology, 67 (4):526-533.

Plane JM, Shen Y, Pleasure DE, Deng W. (2010) Prospects for minocycline neuroprotection. Arch Neurol, 67 (12), 1442-1448.

Deng W. (2010) Exploiting pluripotency for therapeutic gain. Panminerva Medica, 52 (2): 167-173. PubMed ID: 20517198

Deng W. (2010) AID in reprogramming: quick and efficient. BioEssays, 32 (5): 385-387.

Shen Y, Plane, JM, Deng W. (2010) Mouse models of periventricular leukomalacia. J Vis Exp, 2010 May 18;(39). pii: 1951. http://www.jove.com/index/Details.stp?ID=1951, doi: 10.3791/1951. PubMed ID: 20485263.

Jiang P, Selvaraj V, Deng W. (2010) Differentiation of embryonic stem cells into oligodendrocyte precursors. J Vis Exp, 2010 May 19;(39). pii: 1960. http://www.jove.com/index/Details.stp?ID=1960, doi: 10.3791/1960. PubMed ID: 20489683.

Jiang P, Rushing SN, Kong C, Fu J, Lieu D, Chan, C, Deng W, Li, R. (2010) Electrophysiological properties of human induced pluripotent stem cells. Am J Physiol Cell Physiol, 298 (3), C486-495.

Deng W. (2010) Induced pluripotent stem cells: paths to new medicines. EMBO reports, 11 (3), 161-165.

Selvaraj V, Plane, JM, Williams AJ, Deng W. (2010) Switching cell fate: the remarkable rise of induced pluripotent stem cells and lineage reprogramming technologies. Trends in Biotechnology, 28 (4): 214-223.

Deng W. (2010) Stem cells and drug discovery: the time has never been better. Trends in BioPharmaceutical Industry, 6 (1): 12-20. (ISSN: 1943-2518).

Selvaraj V, Soundarapandian MM, Chechneva O, Williams AJ, Sidorov MK, Soulika AM, Pleasure DE, Deng W. (2009) PARP-1 deficiency increases the severity of disease in a mouse model of multiple sclerosis. J Biol Chem, 284 (38), 26070–26084.

Deng W. (2009) PARylation: strengthening the connection between cancer and pluripotency. Cell Stem Cell, 5: 349-350.

Deng W*, Xu Y*. (2009) Genome integrity: linking pluripotency and tumorgenicity. Trends in Genetics, 25 (10): 425-427. *Co-correspondence authors.

Deng W, Pleasure J, Pleasure D. (2008) Progress in periventricular leukomalacia. Arch Neurol, 65 (10), 1291-1295.

Deng W, Neve RL, Rosenberg PA, Volpe JJ, Jensen FE. (2006) AMPA receptor composition and CREB regulate oligodendrocyte excitotoxicity. J Biol Chem, 281, 36004-36011.

Deng W, Yue Q, Rosenberg PA, Volpe JJ, Jensen FE. (2006) Oligodendrocyte excitotoxicity determined by local glutamate accumulation and mitochondrial function. J Neurochem 98, 213-222.

Sanchez RM, Deng W, Levada RE, Lippman JJ, Jensen FE. (2005) AMPA/kainite receptor-mediated downregulation of GABAergic synaptic transmission by calcineurin after seizures in the developing rat brain. J Neurosci. 25, 3442-3451.

Deng W, Wang H, Rosenberg PA, Volpe JJ, Jensen FE. (2004) Role of metabotropic glutamate receptors in oligodendrocyte excitotoxicity and oxidative stress. Proc Natl Acad Sci USA 101, 7751-7756.

Follett PL, Deng W, Talos D, Massillon L, Rosenberg PA, Volpe JJ, Jensen FE. (2004) Glutamate receptor-mediated oligodendrocyte toxicity in periventricular leukomalacia: a protective role for topiramate. J Neurosci 24, 4412-4420.

Deng W, Rosenberg PA, Volpe JJ, Jensen FE. (2003) Calcium-permeable AMPA/kainate receptors mediate toxicity and preconditioning by oxygen-glucose deprivation in oligodendrocyte precursors. Proc Natl Acad Sci USA 100, 6801-6806.

Rosenberg PA, Deng W, Gan XD, Ali S, Fu J, Back SA, Sanchez RM, Segal MM, Follett PL, Jensen FE, Volpe JJ. (2003) Mature myelin basic protein-expressing oligodendrocytes are insensitive to kainate toxicity. J Neurosci Res. 71, 237-245.

Deng W, Poretz RD. (2003) Oligodendroglia in developmental neurotoxicity. Neurotoxicology 24, 161-178 (featured with cover illustration).

Deng W, Poretz RD. (2002) Protein kinase C activation is required for the lead-induced inhibition of proliferation and differentiation of cultured oligodendroglial progenitor cells. Brain Res 929, 87-95.

Deng W, Poretz RD. (2001) Chronic dietary lead exposure affects galactolipid metabolic enzymes in the developing rat brain. Toxicol Appl Pharmacol 172, 98-107.

Deng W, McKinnon RD, Poretz RD. (2001) Lead exposure delays the differentiation of oligodendroglial progenitors in vitro. Toxicol Appl Pharmacol 174, 235-244.

Deng W, Poretz RD. (2001) Lead alters the developmental profile of galactolipid metabolic enzymes in cultured oligodendrocyte lineage cells. Neurotoxicology 22, 429-437.

Poretz RD, Yang A, Deng W, Manowitz P. (2000) The interaction of lead exposure and arylsulfatase A genotype affects sulfatide catabolism in human fibroblasts. Neurotoxicology 21, 379-388.