Basic Science Microscopy Core
This facility is a multi-user, interdepartmental resource that enables local scientists to implement confocal techniques in their research programs.
Olympus FluoView FV1000 spectral confocal system
The system is capable of simultaneous collection from up to 4 detection channels using multiple laser and conventional illumination sources. The FV1000 has 2 laser scanners that allow simultaneous confocal fluorescence observation and independent laser light stimulation. Emission bandwidths can be automatically set for each fluorochrome. In addition, the spectral detector’s variable bandwidth allows the fluorescence emission bandwidth to be manually optimized for each fluorescent dye. Spectral unmixing can also be carried out. The system is interfaced with an Olympus 1X81 inverted microscope that is fitted with dry, oil and water immersion objectives (10x, 20x, 40x, and 60x). The microscope stands on an anti-vibration table.
- Confocal fluorescence imaging. Multicombiner Laser enabling excitation wavelengths ranging from 405-635 nm
- Brightfield and Nomarski DIC imaging
- Spectral detection
- Other imaging modes include time-lapse, depth, FRAP (fluorescence recovery after photobleaching) and FRET (fluorescence resonance energy transfer.